NETs formation and cell-free DNA were found to be increased in sepsis patient plasma supernatants, with the rise in IL-8, IL-10, IL-18, etc., and a decline in IL-1β, IL-2, IL-4, etc. cytokines and DNAJC13, GPI, GSTP1, PNP, ANPEP, COTL1, GCA, PGAM1, TBMBS4X, and APOA1 proteins which play important roles in neutrophil activation and its immune response. The gene discussed is COTL1; the disease is Sepsis.