In summary, co-stimulation with wheat amylase trypsin inhibitors and gliadin could exacerbate the clinical symptoms and temperature associated with celiac disease in BALB/c mice, aggravating the intestinal villi atrophy, crypt hyperplasia and inflammatory cell infiltration of mice, as well as overexpressing tTG and IL-15 in intestinal tissues and shifting the balance of Th1/Th2 lymphocyte subsets toward Th1 immunity in the spleen. Here, TGM2 is linked to celiac disease.