Our study shows that an inhibitor of the PI3K/Akt/mTOR pathway, MK-2206, suppressed PI3K/Akt/mTOR pathway activation and abolished the EMT, CSC stemness, and invasion induced by CXCR4, most likely because the PI3K/Akt/mTOR pathway plays an important role in tumor cell EMT behavior and CSC stemness regulation in EOC cells. The gene discussed is AKT1; the disease is neoplasm.