To further confirm the effect of the PI3K/Akt/mTOR signaling pathway in CXCR4-induced EMT and CSC stemness in vivo, we used WB and immunofluorescence (IF) to investigate CXCR4-, EMT- and CSC-related protein expression, specifically the Akt signaling pathway, in CXCR4-kD#2/OVCA420 and CXCR4/SKOV3 tumor tissues derived from xenograft tumor nude mice treated with PTX or control. Here, AKT1 is linked to neoplasm.