To assess if AAV2 delivery of CYP4V2 could enhance CYP4V2 expression in the BCD RPE cells, we transduced BCD iPS-RPE cells with either AAV2.wtCYP4V2 or AAV2.coCYP4V2, followed by analysis of Western blot, immunocytochemistry, and Multi-CYP functional assay. This evidence concerns the gene PPIG and Bietti crystalline dystrophy.