Importantly, the downregulation of ALG10 will destroy the binding ability of TGFBR2 to TGFBR1, further weaken the phosphorylation of Smad2 and thus inactivate TGF-β signaling inactivation, finally inhibiting the CSC-like characteristics of CRC cells. This evidence concerns the gene TGFB1 and colorectal carcinoma.