At the salivary gland infection stage, CpA-Dcr2 mosquitoes displayed a significant 6-fold reduction in ZIKV infection intensity (Fig 2F, Mann–Whitney test, ***P < 0.001) and 1.7-fold reduction in prevalence (Fig 2F, Fisher’s exact test, ****P < 0.0001), and CpA-R2d2 mosquitoes showed a significant 5.8-fold reduction in ZIKV infection intensity (Fig 2F, Mann–Whitney test, **P < 0.01) and 1.5-fold reduction in prevalence (Fig 2F, Fisher’s exact test, ****P < 0.0001). Here, CPA1 is linked to Zika virus infectious disease.