CPA1 and Zika virus infectious disease: To investigate whether the transgenic up-regulation of Dcr2 or R2d2 that mediated suppression of the microbiota also influences antiviral activity, we performed identical DENV2 and ZIKV infection assays with the CpA-Dcr2, CpA-R2d2, and WT mosquitoes under near germ-free conditions (or here called aseptic conditions, achieved through antibiotic treatment), then assayed viral titers in the midguts at 7 dpi and salivary glands at 14 dpi.