At the G4 generation, all 9 heterozygous lines were infected with both DENV serotype 2 (DENV2) and ZIKV; the CpA-Dcr2-L2 (line #2) and CpA-R2D2-L2 (line #2) displayed the strongest resistance to both DENV2 and ZIKV, with lower infection intensity and prevalence (S1 and S2 Figs). Here, CPA1 is linked to infection.