CPA1 and arbovirus infection: To investigate the impact of blood meal-induced overexpression of Dcr2 and R2D2 on arbovirus infection, we infected CpA-Dcr2 and CpA-R2d2 transgenic mosquitoes, along with sibling WT (referred to as WT onwards) as control, with DENV2, ZIKV, and CHIKV through artificial blood meals containing 106–7, 108, and 107–8 PFU/ml virus particles, respectively (Fig 2), and then measured the infection intensity and prevalence in the midgut at 7 days post-infection (dpi) and the dissemination rate in the carcass and salivary glands at 14 dpi.