Furthermore, hsa-mir-324 could directly interact with and inhibit the expression of SOD2 in CRC cells, while 4-AAQB significantly inhibited the cell viability of the human CRC cell lines DLD1 and HCT116 by weakening their proliferation, migration, invasion, and clonogenic ability, and inhibited the tumorigenicity of CRC. The gene discussed is SOD2; the disease is colorectal carcinoma.