TLR2 and infection: The results showed that L. garvieae colonies could be isolated at 6, 12, 24, and 48 hours after infection with planktonic and biofilm bacteria (Table 5).For the TLR2, IL-1β gene, we found that it was upregulated at 6, 12, and 24 hours and downregulated at 50 hours after infection in the planktonic group.