These results indicate that the GCis formula may inhibit the expression of Cxcr2, Ccl17, and Lcn2; promote the expression of PLA, Calm1, and Mylk3; and participate in the regulation of chemokine signaling pathway, platelet activation, and amebiasis signaling pathway to improve the intestinal barrier function. This evidence concerns the gene MYLK3 and amebiasis.