In HT29 and HCT116 cell xenograft tumor tissues, the immunohistochemical results showed that the expression levels of the autophagy marker proteins p53 and Beclin1 were increased in the Rh4-treated group compared with the control group, while the expression levels of p62 decreased, indicating that the autophagy level in the Rh4-treated group increased compared with the control group. This evidence concerns the gene BECN1 and neoplasm.