To clarify whether JNK was activated by IRE1α and subsequently activated autophagy in HCC cells, SMMC-7721 and Hep3B cells were transfected with IRE1α siRNA (si-IRE1α) or negative control siRNA (si-NC) and then incubated with 100 μM myricetin or vehicle for 24 h. This evidence concerns the gene ERN1 and hepatocellular carcinoma.