In the same manner, 27 days after xenograft of the OICI-EPS-0486 organoids (PDO) into a NSG mouse, the tumor developed (ODX) (Supplementary Figure 3D).With the RT-PCR, no INI-1 expression was detected in the OICI-EPS-0530 tumors similar to OICI-EPS-0486 (Supplementary Figure 3A), in contrast to Hela and an Ewing sarcoma cell line, Kamui-EWS, which was used as a positive control (Figure 2F and Supplementary Figures 2A–E). This evidence concerns the gene EWSR1 and Ewing sarcoma.