Collection of blood samples in these patients following MRgFUS mediated BBB/BTB disruption and patients with Alzheimer’s disease as control group for liquid biopsy.Outcomes:This technique enhances the signal for circulating brain-derived biomarkers (plasma cfDNA, neuron-derived extracellular vesicles, and brain-specific protein S100b).cfDNA-mutant copies of isocitrate dehydrogenase 1 (IDH-1) were increased. Here, PROS1 is linked to early-onset autosomal dominant Alzheimer disease.