CD8A and neoplasm: In the present work, we developed a differentiation protocol of central memory cells by in vitro activation of polyclonal total CD8 T lymphocytes from naïve mice that resembles in vivo generated memory cells according to molecular (Figures 2–4), phenotypic (Figures 1, 7, and Supplementary Figure 2) and functional (Figure 12 and Supplementary Figure 2) criteria, including longevity (Figure 5), plasticity (Figures 10, 11) and long-term protection of naïve hosts against lethal bacterial challenge and tumor growth upon transfer (Figure 12).