In the studies on the recurrence and evolution of gliomas by (12, 13) exome sequencing and DNA methylation data were derived from 23 and 19 patients before and after recurrence, respectively, for comparative analysis, from which the researchers discovered intratumoral heterogeneity during initial growth and subclones sharing epigenetic [glioma CpG island methylator phenotype (G-CIMP)] alterations, TP53 and ATRX mutations, and copy number alterations (12, 13). The gene discussed is ATRX; the disease is central nervous system cancer.