CSF3 and limb ischemia: In those continued clinical observations, we focused on the most pressing questions, including 1) is autologous transplantation of the BM-MNCs safe in patients with CLI, 2) how much simultaneous injection of granulocyte-colony stimulation factor (G-CSF) with the transplantation of BM-MNCs would increase the efficacy of cell therapy in CLI patients, 3) can interval implantation of the BM-MNCs improve the treatment efficacy, and 4) can the BM-MNCs interval injection increase collateral vessel formation in patients with limb ischemia?