CCND1 and nonpapillary renal cell carcinoma: CRISPRi-mediated inhibition of E11:69419 led to downregulation of both of these genes—as determined by quantitative PCR with reverse transcription (RT–qPCR) (Fig. 2h)—but only CCND1 was required for ccRCC cell proliferation and tumour formation in vivo (Fig. 2i and Extended Data Fig. 6a, b).