CD133, Nestin and SOX2 expression were positively associated with ITGB8 expression in TCGA and Chinese Glioma Genome Atlas (CGGA) databases (Fig. 1I and S1c), and we further confirmed that ITGB8 was closely correlated with Nestin expression in GBM specimens (Fig. 1J). This evidence concerns the gene NES and central nervous system cancer.