By querying the McPAS-TCR database and setting the maximum Levenshtein distance (the edit distance between two sequences) at 1, we discovered that the first two had a similar sequence derived from PBMC samples of patients with cancer18 19 and that the sequence CASSLGNQPQHF was previously reported in breast and colon cancer CD8+ TIL cells.20 21 Next, we performed the cross-sample clustering analysis based on the reference library generated using the tumor RNAseq data in TCGA-HNSC. Here, CD8A is linked to malignant colon neoplasm.