Dual-luciferase reporter gene experiments confirmed that the luciferase activity of CRC cells co-transfected with the luciferase plasmids psiCHECKTM-2-CCBE1-WT and miR-1205 mimics was markedly reduced compared with the control group, while no obvious change in the luciferase activity of the cells was observed in the cells co-transfected with CCBE1-MUT and miR-1205 mimics (Fig. 5B). This evidence concerns the gene CCBE1 and colorectal carcinoma.