On the one hand, JB causes DNA damage in gastric cancer MKN45 cells and induces the S cycle arrest by activating the ATR-CHK1-CDC25A-Cdk2 signaling pathway, On the other hand, JB induces MKN45 cells apoptosis through the mitochondrial pathway, and ultimately effectively inhibits the growth of gastric cancer cells. This evidence concerns the gene CDC25A and gastric cancer.