ACTA2 and carcinoma: We examined the spatial expression of marker genes previously reported for common cell types in two samples to assess the sensitivity of the method of detecting the transcripts per spot, and the results confirmed that ALB and CYP2E114 were highly expressed in para-carcinoma regions; GPC326 and AKR1B1027 in carcinoma regions; ACTA2 and COL1A1 (markers typically associated with activated fibroblasts or cancer-associated fibroblasts) 28 in the fiber cord and stromal regions, and the above also confirmed the reliability of ST sequencing results.