To further address the cooperation of protease targeting and PI3K inhibition, two inducible Metap1, Metap2 or Usp7 knockdown breast cancer cell lines (PyB6-TA, PyMG-TA) and one non-cancerous normal murine mammary gland cell line (NMuMG/E9-TA) were used. This evidence concerns the gene PIK3CA and breast carcinoma.