Because the synergistic effects might be due to a direct link of METAPs to PI3K signaling, we analyzed the activity of the PI3K pathway by means of the phosphorylation status of three players (Akt, mTOR, S6) upon METAP1 or METAP2 knockdown in human breast cancer cells (Supplementary Figure S16). This evidence concerns the gene PIK3CA and breast carcinoma.