Consistently, the clinical data analysis using TCGA-PAAD indicated that high JAG1 expression correlated with poor survival, while high expressions of NOTCH1, NOTCH2, NOTCH3, and DLL4 did not affect survival (Figure 6D and Figure S5B). These results suggest that the NOTCH2/JAG1 pathway between EC and PDAC plays an important role in the plasticity of cancer cells, and NOTCH2/JAG1 pathway could have caused poor survival by tumor heterogeneity. This evidence concerns the gene NOTCH3 and pancreatic adenocarcinoma.