Based on the above promising results, the therapeutic effect of RAP@T/R NPs was evaluated in a pathological atherosclerosis model in ApoE-/- mice, following the treatment protocol shown in Figure 6A. After 12 weeks of high-fat feeding, the average weight of mice in each treatment group increased from about 22 grams to 31 grams, and there was no significant difference among the groups treated with RAP@T/R NPs, RAP@NPs, free RAP, and saline (Figure 6B). This evidence concerns the gene APOE and atherosclerosis.