To clarify the involvement of EV miR-144 in CIH E-EV-induced endothelial dysfunction, we demonstrated that CIH E-EV treatment increased miR-144 expression and reduced NRF2 expression, increased superoxide production in endothelial cells, attenuated aorta EDR, and increased systolic blood pressure. This evidence concerns the gene NFE2L2 and endothelial dysfunction.