CD274 and colorectal carcinoma: To determine whether the kinase activity of PIPKIγ is required for its function in modulating PD-L1 and CRC proliferation or not, we overexpressed kinase-dead PIPKIγ (PIPKIγ-KD) in HCT116 and SW620 cells (with lower intrinsic PIPKIγ expression) and evaluated PD-L1 expression and CRC proliferation (Figure S3A).