Considering cancer immunotherapy with ICB is based on the inhibition of critical immune checkpoints, we evaluated some representative molecules and found that PD-1, PD-L1, CTLA-4, and TIGIT were extensively elevated in the AMES > 4 group (Figure 6I), as well as the CYT score which is used to reflect the cytotoxic effects (Figure 6J). The gene discussed is CTLA4; the disease is cancer.