To further support the functional activation of T lymphocytes by CML-RAE-1γ-Dex, in addition to flow cytometric detection, ELISPOT assays were used to assess the secretion of cytotoxic cytokines including TNF-α and IFN-γ [51], and ELISA was performed to assess the production of IL-2, a critical cytokine that is synthesized by activated T cells [51]. Here, TNF is linked to chronic myelogenous leukemia, BCR-ABL1 positive.