The results of WB analysis showed that EPI suppressed the expression of epithelial markers (E-cadherin), enhanced the expression of mesenchyme markers (N-cadherin, vimentin) and CSC markers (CD133, CD44) in CRC cells (Fig. 2N, O), indicating that EPI promotes EMT and CSCs in CRC cells. This evidence concerns the gene CD44 and colorectal carcinoma.