Through KO and SSA vector construction, lentivirus packaging, infecting the target cells and monoclonal screening, stable ovarian cancer cell lines with EEF1D KO were successfully established, in which the KO of the EEF1D gene was validated by sequencing the target gene and Western blotting in SKOV3 and SKOV3/DDP cells (Fig. S3). The gene discussed is EEF1D; the disease is ovarian cancer.