By constructing the pLJM1-shRNA EEF1D plasmid, lentivirus packaging, infecting the target cells and subculturing cells under puromycin screening, the four stable ovarian cancer cell lines (SKOV3/EEF1D shRNA, SKOV3/ scrambled shRNA, SKOV3/DDP/EEF1D shRNA and SKOV3/DDP/scrambled shRNA) were successfully established, among which SKOV3/scrambled shRNA and SKOV3/DDP/scrambled shRNA cell lines were used as the controls. This evidence concerns the gene EEF1D and ovarian carcinoma.