The downregulated pathways of HCC-186 were majorly enriched by the genes such as IKBKE, PDGFB, IL12A, IL1A, IRAK1, ITGB2, MYC, CALML4, ADCY7, TLR4, CALML5 and IL1B. This evidence concerns the gene IKBKE and hepatocellular carcinoma.