Flow cytometry analysis was conducted to determine the CD133 and CD44 positive cell ratio.<h4>Results</h4>We evaluated the relative expression of ATAD2 in four lung cancer cell lines (A549, SPC-A1, H460, and H1299 cells) and found increased mRNA and protein levels of ATAD2 in lung cancer samples. Here, ATAD2 is linked to lung carcinoma.