Fibroblast clusters 2 (FB2) and 4 (FB4), which were increased in the early phase after myocardial infarction, showed lower expression levels of Htra3 (Supplementary Fig. 6d–f) and the total expression levels of Htra3 were decreased at the early phase after MI (Supplementary Fig. 6g), which are consistent with the finding that expression of Htra3 in cardiac fibroblasts was down-regulated after pressure overload to the heart (Fig. 2e) or mechanical stretch (Fig. 2g). This evidence concerns the gene HTRA3 and myocardial infarction.