We found that the methylation level of PAX8 in endometrial carcinoma tissues was significantly lower than that of normal controls, and the TP53 mutant samples also showed the lowest methylation level and the highest transcription level (Fig. 2D–F), and this kind of tendency was consistent with changes in p53-related pathways (Fig. 2G). The gene discussed is TP53; the disease is endometrial carcinoma.