Mecp2+/- mice and Mecp2+/+ littermate controls were treated with either vehicle (10% tween 80) or the M1-PAM VU595 and progressed through a battery of tests encompassing the major RTT symptom domains, including open field (motor and anxiety), three-chamber social interaction (social), novel object recognition (spatial memory), and contextual fear conditioning (associative memory). Here, MECP2 is linked to Anxiety.