We overexpressed DPEP1 in HCT116 and RKO cells, respectively, and then detected the expression of other colon cancer stem cell markers by western blotting, which demonstrated that the expression levels of ASCL2, CD133, CD44, LGR5, and NKD1 were markedly increased in the cells transfected with pCMV‐DPEP1 relative to the cells transfected with pCMV (Figure 5A), which suggested that DPEP1 could enhance the stemness of colon cancer cells. This evidence concerns the gene PROM1 and malignant colon neoplasm.