To verify the function of Gpr174-/- BMDCs in alleviating DSS-induced colitis, we adoptively transfer Gpr174-/- BMDCs and Gpr174+/+ BMDCs into C57/BL6 mice intravenously at 5 days and 3 days before and 1 day after the induction of colitis, flow chart of procedure were shown in Figure 6C. Here, GPR174 is linked to colitis.