In order to verify the relationship between PD-L1 and IRAK1, we used flow cytometry to detect PD-L1 expression in tumor cells treated with different concentrations of pacritinib and found that the level of PD-L1 upregulated, suggesting that IRAK1 correlated with PD-1/PD-L1 axis and mediated immunosuppression. The gene discussed is CD274; the disease is neoplasm.