Reduced dimensionality (t-SNE) visualization corroborated unbiased clustering of these cases within the “IDH-mutant High-Grade Astrocytoma” group and away from all IDH-wildtype GBM groups (“GBM, RTK I”; “GBM RTK II”; “GBM RTK III”; “GBM-MYCN”; “GBM-MES”; “GBM-MID”; “GBM-K27”; “GBM-G34”) (Figure 2). The gene discussed is IDH1; the disease is astrocytoma (excluding glioblastoma).