Besides, berberine down-regulates the expression of acetylcholinesterase and inhibits acetylcholinesterase activity in Alzheimer’s disease model mice hippocampus which induced by heavy metals, in addition, berberine normalizes the production of inflammatory factors, such as tumor necrosis factor-alpha (TNF-α), IL-6, IL-1β (Hussien et al., 2018). The gene discussed is ACHE; the disease is Alzheimer disease.