In present research, we also found that survivin inhibition affected the expression of above-mentioned proteins related to proliferation (PI3K, Akt, pAkt), apoptosis (Bcl-2, Bax, Caspase3/9) and metastasis (MMP2, MMP9, VEGF, Snail, E-cadherin, Vimentin) in ESCC (Figure 6), which further demonstrated that survivin plays a significant role in ESCC. This evidence concerns the gene MMP2 and esophageal squamous cell carcinoma.