To investigate the mechanism of DSE against ESCC cell proliferation, we detected the effects of DSE on PI3K/Akt pathway and observed the decreased PI3K, Akt and pAkt proteins levels in KYSE450 and Eca109 cells administrated with different concentration of DSE (Figures 2H,I). This evidence concerns the gene AKT1 and esophageal squamous cell carcinoma.