TOP1 and chronic leukemia: These included evaluation of antibacterial activity toward Staphyloccocus aureus, S. epidermidis, and Escherichia coli, cytotoxic activity against three human tumor cell lines, including human promyelocytic leukemia HL-60, human lung carcinoma A-549, chronic leukemia K562 cell lines and one human normal liver cell line HL7702 (L-02), DNA topoisomerase I (Topo I), and acetylcholinesterase (AChE) inhibitory activities.