IFNG and cancer: Neoantigen-specific T cell response, assessed by IFNγ ELISpot performed on splenocytes collected at day 23, was significantly increased over the control mice after cotreatment with plasmid M8 and αCTLA-4 or both ICIs, but not in mice cotreated with plasmid M8 and αPD-1, thus suggesting a synergistic effect of αCTLA-4 and plasmid M8 vaccine in eliciting an antitumoral effect in this cancer model.