In the tumor-bearing mice infected with P. yoelii, we found an increased proportion of TUNEL-positive cells, upregulated expression of proapoptotic factors including Bax, caspase-9, and cleaved caspase-3, and downregulated expression of antiapoptotic factor Bcl-2 in colon cancer tissues, indicating that Plasmodium infection promoted mitochondria-mediated apoptosis. Here, CASP3 is linked to malignant colon neoplasm.