In the two pleural effusion samples, the cytokines with the highest concentrations were IL-1ra (40,735.95 pg/ml for Gp4-8-2021, 9016.76 pg/ml for Gp4-9-2021) and IL-18 (3237.45 pg/ml for Gp4-8-2021 and 2764.82 pg/ml for Gp4-9-2021) (Table 4). The gene discussed is IL18; the disease is Pleural effusion.