For the 1.34 Mb capture, we found that tumors with reconstruction error >39% or carrying <9 somatic mutations were unlikely to generate a SBS18/36 TMS profile that was caused by biallelic inactivation of MUTYH. These measures are negatively correlated (ρ = −0.41) and exclude tumors for different reasons: the constraint on minimum somatic mutations reflects our previous finding that MUTYH positive CRCs exhibit significantly higher tumor mutational burden (TMB) than MUTYH negative mismatch repair (MMR)-proficient tumors7, confirmed by this larger study. This evidence concerns the gene MUTYH and neoplasm.