To identify redox vulnerabilities for KEAP1/NRF2 mutant NSCLC, we conducted a CRISPR-Cas9-based negative selection screen for antioxidant enzyme genes whose loss sensitized cells to sub-lethal concentrations of the superoxide (O2•–) -generating drug β-Lapachone. This evidence concerns the gene NFE2L2 and non-small cell lung carcinoma.