Using a tumor cell loaded probe to bring the cancer cell into contact with ECs via AFM cantilever approach/retraction cycles, we have analyzed real-time adhesion events between MB231 and ECs in three different EC confluence states (NCF, SCF and CF) and Mgrt state in a scratch assay for three periods (0.5, 10 and 60 sec) of cell-cell contact. This evidence concerns the gene KITLG and cancer.