Therefore, we generated and confirmed two clonal CRISPR/Cas9 knockout lines (PFN1(-/-)) in immortalized neuroblastoma-2a (N2a) cells (Figure 6A and Figure 6—figure supplement 1A-C) that have been successfully used to model features of ALS (De Vos, 2007; Vance et al., 2009; Coussee et al., 2011; Henty-Ridilla et al., 2017). This evidence concerns the gene PFN1 and amyotrophic lateral sclerosis.